From Biochemical Assays in Cells to Applications in Ranges: An Antidotal Treatment of Intoxication by Pussley (Portulaca oleracea)
|
| Title | From Biochemical Assays in Cells to Applications in Ranges: An Antidotal Treatment of Intoxication by Pussley (Portulaca oleracea) |
| Authors | |
| Abstract | The assessment of the cytotoxicity of pussley (Portulaca oleracea L.) enrolls in the first series of studies of ex vivo evaluation of toxicity of range plants in Palestine. In parallel, this study looks for an antidotal treatment in Milk Thistle (Silybum marianum) which is available in the Palestinian environment. This study has an economical, ecological, agricultural and political importance through preserving livestock, ranges and land…Starting at 500 µg of Portulaca oleracea extract/ml of HepG2 cell culture medium, a clear toxicity for HepG2 cell line as expressed by MTT viability test is demonstrated as directly proportional to the plant dose. Silybum marianum shows a similar profile even though with a viability profile weaker than that of Portulaca oleracea. This is unexpected for a plant (Silybum marianum) used classically as hepatic regenerative and antidote agent, recall that Portulaca oleracea is an edible plant.However, Silybum marianum is demonstrated to be efficient (at 1.5 mg of plant extract/ml of HepG2 cell line culture medium) as an antidote against the toxicity of Portulaca oleracea. This opens horizons for using Silybum marianum as an antidote in case of intoxication of livestock and also humans. |
| Publisher | World Science Publisher |
| Date | 2013-05-26 |
| Source | 2167-0854 |
| Rights | Copyright NoticeProposed Creative Commons Copyright Notices1. Proposed Policy for Journals That Offer Open AccessAuthors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work´s authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal´s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).Proposed Policy for Journals That Offer Delayed Open AccessAuthors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication, with the work [SPECIFY PERIOD OF TIME] after publication simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work´s authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal´s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access). |