WIL-2 NS LYMPHOMA CELL LINE SHOW APOPTOTIC FEATURES WHEN TREATED WITH TRADITIONAL MEDICINE COMMELINA BENGHALESIS
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| Title | WIL-2 NS LYMPHOMA CELL LINE SHOW APOPTOTIC FEATURES WHEN TREATED WITH TRADITIONAL MEDICINE COMMELINA BENGHALESIS |
| Authors | |
| Abstract | Many natural occurring agents have shown chemo preventive and chemotherapy potentials, yet there are no effective drugs to treat most cancers. Commelina benghalensis has been traditionally used as a herbal plant whose leaves are believed to have anti-tumour properties. The aim of this study was to investigate the effect of semi-purified extracts from C. benghalensis on the proliferation of Wil-2 NS cells. An acetone extract was prepared from the leaves and defatted with n-hexane and fractionated with dichloromethane (F2). F2 was tested for anti-proliferative activities on Wil-2 NS cells. Results from the trypan blue exclusion and sulphorhodamine B assays showed F2 inhibited the proliferation of the Wil-2 NS cells in a dose and time dependent manner. The acridine orange/ethidium bromide dual stain showed that the treated cells die through a process of apoptosis. These results show that C. benghalesis has anti-proliferation properties against Wil-2NS lymphoma cells. Further studies are required to determine the components responsible from the extracts and elucidate the mechanism of action on growth inhibition. Acknowledgements: NRF and University of Limpopo Research office. |
| Publisher | African Ethnomedicines Network |
| Date | 2009-06-07 |
| Source | African Journal of Traditional, Complementary and Alternative medicines (AJTCAM) ABSTRACTS OF THE WORLD CONGRESS ON MEDICINAL AND AROMATIC PLANTS, CAPE TOWN NOVEMBER 2008 |
| Rights | Articles can be used for educational and non-comercial purposes. The copyrights of articles are retained by the authors with publication rights granted to AJTCAM. |