Identification of DNA Elements Involved in Somaclonal Variants of Rauvolfia Serpentina (L.) Arising from Indirect Organogenesis as Evaluated by ISSR Analysis
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| Title | Identification of DNA Elements Involved in Somaclonal Variants of Rauvolfia Serpentina (L.) Arising from Indirect Organogenesis as Evaluated by ISSR Analysis |
| Authors | |
| Abstract | Rauvolfia serpentina an endangered medicinal plant was chosen for in vitro propagation using modified MS medium. Genetic fidelity study of the regenerated plants were analysed with 18 ISSR markers. A total of 159 monomorphic bands were obtained; one ISSR marker HB-12 showed a polymorphic band among the callus regenerants. The specific polymorphic fragment was then gel eluted, cloned and sequenced. |
| Publisher | Indian Society for Education and Environment (ISEE) |
| Date | 2011-10-01 |
| Source | Indian Journal of Science and Technology Volume 4, Issue 10, October 2011 |