Characterization of Immobilized Laccase from γ-proteobacterium JB: Approach towards the Development of Biosensor for the Detection of Phenolic Compounds
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| Title | Characterization of Immobilized Laccase from γ-proteobacterium JB: Approach towards the Development of Biosensor for the Detection of Phenolic Compounds |
| Authors | |
| Abstract | The two factors important when optimization of enzyme immobilization for the fabrication of biosensor are: activity and stability. The present study investigates the 2 factors when laccase was immobilized on various supports by different methods. Immobilization of partially purified laccase showed that enzyme expressed 100% activity when immobilized on the nitrocellulose membrane. pH and temperature optimum of immobilized laccase was 6.5 and 55°C respectively, when syringaldazine was used as a substrate. Immobilized laccase on nitrocellulose membrane was 100% stable at 4°C -30°C for three months. At 60°C enzyme showed 50% stability after 30 min. Immobilized laccase showed best response with syringaldazine which gave reaction even at 1 to 5μM concentration. Immobilized laccase gave response to catechol, catechin and L-methyl DOPA in the range of 40 to 90, 40 to 60, 30 to 70 µM respectively. ABTS (2,2´-azino-bis (3-ethylbenzothiazoline-6-sulfonate)) also showed response to the laccase from 0.1 to 0.2 mM. |
| Publisher | Indian Society for Education and Environment (ISEE) |
| Date | 2010-01-01 |
| Source | Indian Journal of Science and Technology Volume 3, Issue 1, January 2010 |